Campopiano Group
Publications
2023
Our profile on Google Scholar can be found here.
Amine Synthesis Using the Amine Donor N-Phenyl Putrescine (NPP) and the Johnson Matthey Transaminase Biocatalyst Library.
Kimberley C. Dodds, Catherine A. McKenna, Beatriz Dominguez, Andrew L. Lawrence, Dominic J. Campopiano.
DOI: https://doi.org/10.1595/205651323X16843361771150
Versatile Chemo-Biocatalytic Cascade Driven by a Thermophilic and Irreversible α-Oxoamine Synthase. B. Ashley, A. Baslé, M. Sajjad, A. el. Ashram, P. Kelis, J. M.Wright, D. J. Campopiano, ACS Sustain. Chem. Eng. 2023, xxx, xxx-xxx
DOI: https://doi.org/10.1021/acssuschemeng.3c00243
Publications
2022
Coupled Natural Fusion Enzymes in a Novel Biocatalytic Cascade Convert Fatty Acids to Amines, Shona M. Richardson, Piera M. Marchetti, Michael A. Herrera, and Dominic J. Campopiano, ACS Catal., 2022
DOI: https://doi.org/10.1021/acscatal.2c02954
BioWF: A Naturally-Fused, Di-Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope
Dr. Shona M. Richardson, Dr. Peter J. Harrison, Michael A. Herrera, Dr. Menglu Wang, Rebecca Verez, Dr. Gustavo Perez Ortiz, Prof. Dr. Dominic J. Campopiano, ChemBioChem, 2022.
DOI: https://doi.org/10.1002/cbic.202200171
Characterization of inositol lipid metabolism in gut-associated Bacteroidetes
Stacey L. Heaver , Henry H. Le , Peijun Tang , Arnaud Baslé, Claudia Mirretta Barone , Dai Long Vu , Jillian L. Waters1 , Jon Marles-Wright, Elizabeth L. Johnson , Dominic J. Campopiano and Ruth E. Ley , Nat. Micor. Biol., 2022
DOI: 10.1038/s41564-022-01152-6
Herrera, M.A., Campopiano, D.J. Buy one, get one free. Nat. Synth 1, 420–421 (2022).
https://doi.org/10.1038/s44160-022-00092-7
Creation of an Engineered Amide Synthetase Biocatalyst by the Rational Separation of a Two‐Step Nitrile Synthetase
Alexis JA Hennessy, Wenli Huang, Chloé Savary, Dominic J Campopiano, ChemBioChem, 2021.
DOI: https://doi.org/10.1002/cbic.202100411
N-Phenylputrescine (NPP): a natural product inspired amine donor for biocatalysis.
Catherine A. McKenna,a Mária Štiblariková,b Irene De Silvestro,c Dominic J. Campopiano *a and Andrew L. Lawrence *a, Green Chem., 2022
DOI: https://doi.org/10.1039/D1GC02387J
Publications
2021
Convergent evolution of bacterial ceramide synthesis
Stankeviciute. G., Tang. P, Ashley. B, Chamberlain J.D, Hansen. M. E. B, Coleman. A,
D’Emilia. R, Fu. L, Mohan. E. C, Nguyen. Hung, Guan. Z, Campopiano. D. J, Klein. Eric A.
https://doi.org/10.1038/s41589-021-00948-7
Direct Monitoring of Biocatalytic Deacetylation Reactions by 1H NMR Reveals Fine Details of Substrate Specificity.
De Cesare S., McKenna C. A., Murray L., Bella J., Mullholland N. P. and Campopiano D. J.,Org. Biomol. Chem.,2021
DOI: https://doi.org/10.1039/D1OB00122A
Porphyromonas gingivalis Sphingolipid Synthesis Limits the Host Inflammatory Response
FG Rocha, ZD Moye, G Ottenberg, P Tang, DJ Campopiano, FC Gibson III, ME Davey, Journal of Dental Research, 2021,
https://doi.org/10.1177/0022034520908784
Publications
2020
D-Phenylglycine aminotransferase (D-PhgAT) – substrate scope and structural insights of a stereo-inverting biocatalyst used in the preparation of aromatic amino acids.
Scerpio A., De Cesare S., Marles-Wright J., Akhtar M. K., Loake G., Campopiano D. J., Cat. Sci. Technol., 2020.
DOI: https://doi.org/10.1039/D0CY01391A
Harnessing and engineering amide bond forming ligases for the synthesis of amides
Winn M., Richardson. S. M., Campopiano D.J., Micklefield J., Curr. Opin. Chem. Biol., 2019.
DOI: 10.1016/j.cbpa.2019.12.004
Publications
2019
Synthesis of N-acyl amide natural products using a versatile adenylating biocatalyst
Marchetti P.M., Richardson. S. M., Kariem N.M., Campopiano D.J., Med. Chem. Comm., 2019.
DOI: 10.1039/C9MD00063A
Use of Isotopically Labelled Substrates Reveals Kinetic Differences Between Human and Bacterial Serine Palmitoyltransferase
Harrison P.J., Gable K., Somashekarappa N., Kelly V., Clarke D.J., Dunn, T.M., Campopiano D.J., J. Lipid Res., 2019.
DOI: 10.1194/jlr.M089367
Publications
2018
Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library
Ekström A.E., Wong J.T., Bella J., Campopiano D.J., Org. Biomol. Chem., 2018, 16, 8144-8149.
DOI: 10.1039/C8OB01918E
Sphingolipid biosynthesis in man and microbes.
Harisson P.J., Dunn T.M., Campopiano D.J.; Nat. Prod. Rep., 2018, 35, 921-954.
DOI: 10.1039/C8NP00019K
The carbon chain-selective adenylation enzyme TamA: the missing link between fatty acid and pyrrole natural product biosynthesis.
Marchetti, P. M.; Kelly, V.; Simpson, J. P.; Ward, M.; Campopiano, D. J.; Org. Biomol. Chem., 2018, 16, 2735-2740.
DOI: 10.1039/C8OB00441B.
Publications
2017
Enzymes for Biocatalysis: Key Concepts, Engineering Principles and Case Studies.
Sanchez-Carron, G.; Campopiano, D. J., Chapter 18, Pp 442-470. in Contemporary Catalysis: Science, Technology, and Applications. Kamer, P. C. J.; Vogt, D.; Thybaut, J., (Eds.). RSC Publishing, 2017, ISBN: 978-1-84973-990-0.
Structural evidence for the covalent modification of FabH by 4,5-dichloro-1,2-dithiol-3-one (HR45).
Ekström, A. G.; Kelly, V.; Marles-Wright, J.; Cockroft, S. L.; Campopiano, D. J., Org. Biomol. Chem., 2017, 15, 6310-6313, DOI: 10.1039/c7ob01396e.
Using the Pimeloyl-CoA Synthetase Adenylation Fold to Synthesise Fatty Acid Thioesters.
Wang, M.; Moynié, L.; Harrison, P. J.; Kelly, V.; Piper, A.; Naismith, J. H.; Campopiano, D. J., Nat. Chem. Biol., 2017, 13(6), 660-667, DOI: 10.1038/nchembio.2361.
Characterisation of homologous sphingosine 1-phosphate lyase (S1PL) isoforms in the bacterial pathogen Burkholderia pseudomallei.
McLean, C.; Marles-Wright, J.; Custodio, R; Lowther, J.; Kennedy, A. J.; Pollock, J.; Clarke, D. J.; Brown, A. R.; Campopiano, D. J., J. Lipid Res., 2017, 58(1), 137-150. DOI: 10.1194/jlr.M071258.
I
L-1beta-induced protection of keratinocytes against Staphylococcus aureus-secreted proteases is mediated by human beta defensin 2.
Wang, B.; McHugh, B.J.; Qureshi, A.; Campopiano, D. J.; Clarke, D. J.; Fitzgerald, J. R.; Dorin, J. R.; Weller, R.; Davidson, D. J., J. Invest. Dermatol., 2017, 137(1), 95-105, DOI: 10.1016/j.jid.2016.08.025.
2016
Characterization of secreted sphingosine-1-phosphate lyases required for virulence and intracellular survival of Burkholderia pseudomallei.
Custódio, R.; McLean, C. J.; Scott, A. E.; Lowther, J.; Kennedy, A.; Clarke, D. J.; Campopiano, D. J.; Sarkar-Tyson, M.; Brown, A. R., Mol. Microbiol., 2016, 102(6), 1004-1019, DOI: 10.1111/mmi.13531.
Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans.
Tuck, L. R.; Altenbach, K.; Ang, T. F.; Crawshaw, A. D.; Campopiano, D. J.; Clarke, D. J.; Marles-Wright, J., Sci. Rep., 2016, 6, 22108, DOI: 10.1038/srep22108.
Determination of Protein Thiol Reduction Potential by Isotope Labeling and Intact Mass Measurement.
Thurlow, S. E.; Kilgour, D. P.; Campopiano, D. J.; Mackay, C. L.; Langridge-Smith, P. R.; Clarke, D.J.; Campbell, C. J., Anal Chem., 2016, 88(5), 2727-2733, DOI: 10.1021/acs.analchem.5b04195.
2015
Molecular Basis of Streptococcus mutans Sortase A Inhibition by the Flavonoid Natural Product trans-Chalcone.
Wallock-Richards, D. J.; Marles-Wright, J.; Clarke, D.; Maitra, A.; Dodds, M.; Hanley, B.; Campopiano, D. J., Chem. Commun., 2015, 51(52), 10483-10485, DOI: 10.1039/C5CC01816A.
Continuous Colorimetric Assay That Enables High-Throughput Screening of N-Acetylamino Acid Racemases.
Sanchez-Carron, G.; Fleming, T.; Holt-Tiffin, K. E.; Campopiano, D. J., Anal. Chem., 2015, 87(7), 3923-3928, DOI: 10.1021/ac5047328.
Insights into the Conformations of Three Structurally Diverse Proteins: Cytochrome c, p53, and MDM2, Provided by Variable-Temperature Ion Mobility Mass Spectrometry.
Dickinson, E. R.; Jurneczko, E.; Pacholarz, K. J.; Clarke, D. J.; Reeves, M.; Ball, K. L.; Hupp, T.; Campopiano, D.; Nikolova, P. V.; Barran, P. E., Anal. Chem., 2015, 87(6), 3231-3238. DOI: 10.1021/ac503720v.
Desalting large protein complexes during native electrospray mass spectrometry by addition of amino acids to the working solution
Clarke, D. J.; Campopiano, D. J., Analyst, 2015, 140, 2679-2686, DOI: 10.1039/C4AN02334J.
2014
Garlic Revisited: Antimicrobial Activity of Allicin-Containing Garlic Extracts against Burkholderia cepacia Complex.
Wallock-Richards, D.; Doherty, C. J.; Doherty, L.; Clarke, D. J.; Place, M.; Govan, J. R.; Campopiano, D. J., PLoS ONE, 2014, 9(12): e112726. DOI:10.1371/journal.pone.0112726.
2013
Triazole biotin: a tight-binding biotinidase-resistant conjugate.
Germeroth, A. I.; Hanna, J. R.; Karim, R.; Kundel, F.; Lowther, J.; Neate, P. G.; Blackburn, E. A.; Wear, M. A.; Campopiano, D. J.; Hulme, A. N., Org. Biomol. Chem., 2013, 11(44), 7700-4. DOI :10.1039/c3ob41837e.
The chemical basis of serine palmitoyltransferase inhibition by myriocin.
Wadsworth, J. M.; Clarke, D. J.; McMahon, S. A.; Lowther, J. P.; Beattie, A. E.; Langridge-Smith, P. R.; Broughton, H. B.; Dunn, T. M.; Naismith, J. H.; Campopiano, D. J., J. Am. Chem. Soc., 2013, 135(38), 14276-85. DOI: 10.1021/ja4059876.
Reconstitution of the pyridoxal 5'-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis.
Beattie, A. E.; Clarke, D. J.; Wadsworth, J. M.; Lowther, J.; Sin, H. L.; Campopiano, D. J., Chem. Commun., 2013, 49(63), 7058-60. DOI: 10.1039/c3cc43001d.
Partial complementation of Sinorhizobium meliloti bacA mutant phenotypes by the Mycobacterium tuberculosis BacA protein.
Arnold, M. F.; Haag, A. F.; Capewell, S.; Boshoff, H. I.; James, E. K.; McDonald, R.; Mair, I.; Mitchell, A. M.; Kerscher, B.; Mitchell, T. J.; Mergaert, P.; Barry, C. E. 3rd; Scocchi, M.; Zanda, M.; Campopiano, D. J.; Ferguson, G. P., J. Bacteriol., 2013, 195(2), 389-98. DOI: 10.1128/JB.01445-12.
The Pyridoxal 5'-Phosphate (PLP)-Dependent Enzyme Serine Palmitoyltransferase (SPT): Effects of the Small Subunits and Insights from Bacterial Mimics of Human hLCB2a HSAN1 Mutations.
Beattie, A.E.; Gupta, S.D.; Frankova, L.; Kazlauskaite, A.; Harmon, J. M.; Dunn, T. M.; Campopiano, D. J., BioMed Res. Int., 2013, 194371, DOI: 10.1155/2013/194371.
Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature.
Arnison, P. G.; Bibb, M. J.; Bierbaum, G.; Bowers, A. A.; Bugni, T. S.; Bulaj, G.; Camarero, J. A.; Campopiano, D. J.; Challis, G. L.; Clardy, J.; Cotter, P. D.; Craik, D. J.; Dawson, M.; Dittmann, E.; Donadio, S.; Dorrestein, P.C.; Entian, K. D.; Fischbach, M. A.; Garavelli, J. S.; Göransson, U.; Gruber, C.W.; Haft, D. H.; Hemscheidt, T.K.; Hertweck, C.; Hill, C.; Horswill, A. R.; Jaspars, M.; Kelly, W. L.; Klinman, J. P.; Kuipers, O. P.; Link, A. J.; Liu, W.; Marahiel, M.A.; Mitchell, D. A.; Moll, G. N.; Moore, B. S.; Müller, R.; Nair, S. K.; Nes, I. F.; Norris, G. E.; Olivera, B. M.; Onaka, H.; Patchett, M. L.; Piel, J.; Reaney, M. J.; Rebuffat, S.; Ross, R. P.; Sahl, H. G.; Schmidt, E. W.; Selsted, M. E.; Severinov, K.; Shen. B.; Sivonen, K.; Smith, L.; Stein, T.; Süssmuth, R. D.; Tagg, J. R.; Tang, G. L.; Truman, A. W.; Vederas, J. C.; Walsh, C. T.; Walton, J. D.; Wenzel, S. C.; Willey, J. M.; van der Donk, W. A., Nat. Prod. Rep., 2013, 30(1), 108-160, DOI: 10.1039/c2np20085f.
2012
An Improved Racemase/Acylase Biotransformation for the Preparation of Enantiomerically Pure Amino Acids.
Baxter, S.; Royer, S.; Grogan, G.; Brown, F.; Holt-Tiffin, K. E.; Taylor, I. N.; Fotheringham, I. G.; Campopiano, D. J., J. Am. Chem. Soc., 2012, 134(47), 19310-19313, DOI: 10.1021/ja305438y.
Bivalent enzyme inhibitors discovered using dynamic covalent chemistry.
Clipson, A. J.; Bhat, V. T.; McNae, I.; Caniard, A. M.; Campopiano, D. J.; Greaney, M. F., Chemistry, 2012, 18(34) 10562-10570. DOI: 10.1002/chem.201201507.
L-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5'-phosphate-thiazolidine adduct.
Lowther, J.; Beattie, A. E.; Langridge-Smith, P. R. R.; Clarke, D. J.; Campopiano, D. J., Med. Chem. Commun., 2012, 3, 1003-1008 DOI: 10.1039/C2MD20020A.
Structural, mechanistic and regulatory studies of serine palmitoyltransferase.
Lowther, J.; Naismith, J. H.; Dunn, T. M.; Campopiano, D. J., Biochem. Soc. Trans., 2012, 40(3), 547-554, DOI: 10.1042/BST20110769.
Ferric ion (hydr)oxo clusters in the "Venus flytrap" cleft of FbpA: Mössbauer, calorimetric and mass spectrometric studies.
Mukherjee, A.; Bilton, P. R.; Mackay, L.; Janoschka, A.; Zhu, H.; Rea, D.; Langridge-Smith, P. R.; Campopiano, D. J.; Teschner, T.; Trautwein, A. X.; Schünemann, V.; Sadler, P. J., J. Biol. Inorg. Chem., 2012, 17(4), 573-588, DOI: 10.1007/s00775-012-0878-z.
2011
Role of a conserved arginine residue during catalysis in serine palmitoyltransferase.
Lowther, J.; Charmier, G.; Raman, M. C.; Ikushiro, H.; Hayashi, H.; Campopiano, D. J., FEBS Lett., 2011, 585(12), 1729-1734, DOI: 10.1016/j.febslet.2011.04.013.
2010
Interaction of human ß-defensin 2 (HBD2) with glycosaminoglycans.
Seo, E. S.; Blaum, B. S.; Vargues, T.; De Cecco, M.; Deakin, J. A.; Lyon, M. ; Barran, P. E.; Campopiano, D. J.; Uhrín, D., Biochemistry, 2010, 49(49), 10486-10495, DOI: 10.1021/bi1011749.
The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein.
Raman, M. C.; Johnson, K. A.; Clarke, D. J.; Naismith, J. H.; Campopiano, D. J., Biopolymers, 2010, 93(9) 811-822, DOI: 10.1002/bip.21482.
Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.
Lowther, J.; Yard, B. A.; Johnson, K. A.; Carter, L. G.; Bhat, V. T.; Raman, M. C.; Clarke, D. J.; Ramakers, B.; McMahon, S. A.; Naismith, J. H.; Campopiano, D. J., Mol. Biosyst., 2010, 6(9), 1682-1693, DOI: 10.1039/c003743e.
Antimicrobial activity of CHIR-090, an inhibitor of lipopolysaccharide biosynthesis, against the Burkholderia cepacia complex.
Bodewits, K.; Raetz, C. R.; Govan J. R.; Campopiano, D. J., Antimicrob. Agents. Chemother., 2010, 54(8), 3531-3533, DOI: 10.1128/AAC.01600-09.
Nucleophilic catalysis of acylhydrazone equilibration for protein-directed dynamic covalent chemistry.
Bhat, V. T.; Caniard, A. M.; Luksch, T.; Brenk, R.; Campopiano, D. J.; Greaney, M. F., Nat. Chem., 2010, 2(6), 490-497, DOI: 10.1038/nchem.658.
Conformational preferences of linear beta-defensins are revealed by ion mobility-mass spectrometry.
De Cecco, M.; Seo, E. S.; Clarke, D. J.; McCullough, B. J.; Taylor, K.; Macmillan, D.; Dorin, J. R.; Campopiano, D. J.; Barran, P. E., J. Phys. Chem. B, 2010, 114(6). 2312-2318, DOI: 10.1021/jp9111662.
Subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins based on catalytic activity.
Clarke, D. J.; Ortega, X. P.; Mackay, C. L.; Valvano, M. A.; Govan, J. R.; Campopiano, D. J.; Langridge-Smith, P.; Brown, A. R., Biochemistry, 2010, 49(6), 1319-1330, DOI: 10.1021/bi901703m.
Synthesis and application of a new cleavable linker for "click"-based affinity chromatography.
Landi, F.; Johansson, C. M.; Campopiano, D. J.; Hulme, A. N., Org. Biomol. Chem., 2010, 8(1), 56-59, DOI: 10.1039/b916693a.
2009
Insights into how nucleotide-binding domains power ABC transport.
Newstead, S.; Fowler, P. W.; Bilton, P.; Carpenter, E. P.; Sadler, P. J.; Campopiano, D. J.; Sansom, M. S.; Iwata, S., Structure, 2009, 9(17), 1213-1222, DOI: 10.1016/j.str.2009.07.009.
Dying and necrotic neutrophils are anti-inflammatory secondary to the release of alpha-defensins.
Miles, K.; Clarke, D. J.; Lu, W.; Sibinska, Z.; Beaumont, P. E.; Davidson, D. J.; Barr, T. A.; Campopiano, D. J.; Gray, M., J. Immunol., 2009, 183(3), 2122-2132, DOI: 10.4049/jimmunol.0804187.
Efficient production of human beta-defensin 2 (HBD2) in Escherichia coli.
Vargues, T.; Morrison, G. J.; Seo, E. S.; Clarke, D. J.; Fielder, H. L.; Bennani, J.; Pathania, U.; Kilanowski, F.; Dorin, J. R.; Govan, J. R. W.; Mackay, L.; Uhrín, D.; Campopiano, D. J., Protein Pept. Lett., 2009, 16(6), 668-676, DOI: 10.2174/092986609788490122.
Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.
Tron, C. M.; McNae, I. W.; Nutley, M.; Clarke, D. J.; Cooper, A.; Walkinshaw, M. D.; Baxter, R. L.; Campopiano, D. J., J. Mol. Biol., 2009, 387(1), 129-146, DOI: 10.1016/j.jmb.2008.12.086.
The external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and HSAN1 mutant mimics.
Raman, M. C.; Johnson, K. A.; Yard, B. A.; Lowther, J.; Carter, L. G.; Naismith, J. H.; Campopiano, D. J., J. Biol. Chem., 2009, 284(25), 17328-17339, DOI: 10.1074/jbc.M109.008680.
Interrogating the Molecular Details of the Peroxiredoxin Activity of the Escherichia coli Bacterioferritin Comigratory Protein Using High-Resolution Mass Spectrometry.
Clarke, D. J.; Mackay, C. L.; Campopiano. D. J.; Langridge-Smith, P.; Brown, A. R., Biochemistry, 2009, 48(18), 3904-3914, DOI: 10.1021/bi900189e.
Preparation of isotopically labelled recombinant beta-defensin for NMR studies.
Seo, E. S.; Vargues, T.; Clarke, D. J.; Uhrín, D.; Campopiano, D. J., Protein Expr. Purif., 2009, 65(2), 179-184, DOI: 10.1016/j.pep.2008.11.007.
Contributions of two UDP-glucose dehydrogenases to viability and polymyxin B resistance of Burkholderia cenocepacia.
Loutet, S. A.; Bartholdson, S. J.; Govan, J. R. W.; Campopiano, D. J.; Valvano, M. A.; J. Microb., 2009, 155(6), 2029-2039, DOI: 10.1099/mic.0.027607-0.
Effective binding and sensing of lipopolysaccharide: combining complementary pattern recognition receptors.
Ganesh, V.; Bodewits, K.; Bartholdson, S. J.; Natale, D.; Campopiano, D. J.; Mareque-Rivas, J. C., Angew. Chem. Int. Ed., 2009, 48(2), 356-360, DOI: 10.1002/anie.200804168.
2008
Analysis and separation of residues important for the chemoattractant and antimicrobial activities of beta-defensin 3.
Taylor, K.; Clarke, D. J.; McCullough, B.; Chin, W.; Seo, E.; Yang, D.; Oppenheim, J.; Uhrin, D.; Govan, J. R.; Campopiano, D. J.; MacMillan, D.; Barran, P.; Dorin, J. R., J. Biol. Chem., 2008, 283(11), 6631-6639, DOI: 10.1074/jbc.M709238200.
Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step.
Gupta, M.; Caniard, A.; Touceda-Varela, A.; Campopiano, D .J.; Mareque-Rivas, J. C., Bioconjug. Chem., 2008, 19(10), 1964-1967, DOI: 10.1021/bc800273j.
Plant host and sugar alcohol induced exopolysaccharide biosynthesis in the Burkholderia cepacia complex.
Bartholdson, S. J.; Brown, A. R.; Mewburn, B. R.; Clarke, D. J.; Fry, S. C.; Campopiano, D. J.; Govan, J. R., Microbiol., 2008, 154, 2513-2521, DOI: 10.1099/mic.0.2008/019216-0
2007
The Structure of Serine Palmitoyltransferase; Gateway to Sphingolipid Biosynthesis.
Yard, B. A.; Carter, L. G.; Johnson, K. A.; Overton, I. M.; Dorward, M.; Liu, H.; McMahon, S. A.; Oke, M.; Puech, D.; Barton, G. J.; Naismith, J. H.; Campopiano, D. J., J. Mol. Biol., 2007, 370, 870-886, DOI: 10.1016/j.jmb.2007.04.086.
A putative gene cluster for aminoarabinose biosynthesis in essential for Burkholderia cenocepacia viability.
Ortega, X. P.; Cardona, S. T.; Brown, A. R.; Loutet, S. A.; Flanagan, R. S.; Campopiano, D. J.; Govan, J. R. W.; Valvano, M. A., J. Bacteriol., 2007, 189, 3639-3644, DOI: 10.1128/JB.00153-07.
Maturation of McjA precursor peptide into active microcin MccJ25.
Clarke, D. J.; Campopiano, D. J., Org. & Biomol. Chem., 2007, 5, 2564-2566. DOI: 10.1039/B708478A.
Covalent dimer species of beta-defensin Defr1 display potent antimicrobial activity against multidrug-resistant bacterial pathogens.
Taylor, K.; McCullough, B.; Clarke, D. J.; Langley, R. J.; Pechenick, T.; Hill, A.; Polfer, N. C.; Campopiano, D. J.; Barran, P. E.; Dorin, J. E.; Govan, J. R. W., Antimicriob. Agents Chemother., 2007, 51, 1719-1724, DOI: 10.1128/AAC.01531-06.
Metals in membranes.
Liang, X.; Campopiano, D. J.; Sadler, P. J., Chem. Soc. Rev., 2007, 36, 968-392, DOI: 10.1039/B617040B. Subject of a RSC Chemical Biology focus article.
Histidine ligands in bacterial metallothionein enhance cluster stability.
Blindauer, C. A.; Razi, M. T.; Campopiano, D. J.; Sadler, P. J., J. Biol. Inorg. Chem., 2007, 12, 393-405, DOI: 0. 1007/ s00775-006-0196-4.
2006
Structural and functional studies of defensin-inspired peptides.
Clarke, D. J.; Campopiano, D. J., Biochem. Soc. Trans., 2006, 34, 251-256, DOI: 10.1042/BST0340251.
Discovery of Glutathione S-Transferase inhibitors using dynamic combinatorial chemistry.
Shi, B.; Stephenson, R.; Campopiano, D. J.; Greaney, M. F., J. Am. Chem. Soc., 2006, 128, 8459-8467, DOI: 10.1021/ja058049y.
Short Oxo-Titanium(IV) bond in bacterial transferrin: a protein target for metalloantibiotics.
Guo, M.; Harvey, I.; Campopiano, D. J; Sadler, P. J., Angew. Chem. Int. Ed., 2006, 45(17), 2644-2654, DOI: 10.1002/anie.200600260.
Suicide inhibition of alpha-oxamine synthases: structures of the covalent adducts of 8-amino-7-oxononanoate synthase with trifluoroalanine.
Alexeev, D.; Baxter, R. L.; Campopiano, D. J.; Kerbarh, O.; Sawyer, L.; Tomczyk, N.; Watt, R.; Webster, S. P., Org. Biomol. Chem., 2006, 4(7), 1209-1212 DOI: 10.1039/B517922J.
Mechanism of alpha-oxoamine synthases: identification of the intermediate Claisen product in the 8-amino-7-oxononanoate synthase reaction.
Kerbarh, O.; Campopiano, D. J.; Baxter, R.L., Chem. Commun., 2006, 1, 60-62, DOI: 10.1039/B511837A.
Characterisation of DEFB107 by mass spectrometry: Lessons from an anti-antimicrobial defensin.
McCullough, B. J.; Eastwood, H.; Clarke, D. J.; Polfer, N. C.; Campopiano, D. J.; Dorin, J. A.; Maxwell, A.; Langley, R. J.; Govan, J. R. W.; Bernstein, S. L.; Bowers, M. T.; Barran, P. E., Int. J. Mass Spec., 2006, 252, 180-188, DOI: 10.1016/j.ijms.2006.01.054.
2005
Temperate bacteriophages DK4 and BcepMu from Burkholderia cenocepacia J2315 are unusual, identical and closely associated with the ET12 lineage.
Langley, R. J.; Kervan, D.; Bartholdson, J.; Campopiano, D. J.; Govan, J. R.W., FEMS Microbiol. Letts., 2005, 45, 349-350, DOI: http://dx.doi.org/10.1016/j.femsim.2005.06.001.
Is it biologically relevant to measure the structures of small peptides in the gas-phase?
Barran, P. E.; Polfer, N. C.; Campopiano, D. J.; Clarke, D. J.; Langridge-Smith, P. R. R.; Langley, R. J.; Govan, J. R. W.; Maxwell, A.; Dorin, J. R.; Millar, R. P.; Bowers, M. T., Int. J. Mass Spec., 2005, 240, 273-284, DOI: 10.1016/j.ijms.2004.09.013.
2004
Structure-activity relationships in defensin dimers.
Campopiano, D. J.; Clarke, D. J.; Polfer, N. C.; Barran, P. E.; Langley, R. J.; Govan, J. R. W.; Maxwell, A.; Dorin, J. R., J. Biol. Chem., 2004, 279, 48671-48679, DOI: 10.1074/jbc.M404690200.
Cloning, expression, purification, crystallisation and preliminary x-ray characterisation of the full-length single stranded DNA-binding protein from the hyperthermophilic organism Aquifex aeolicus.
Clarke, D. J.; Northey, C. G.; Mack, L. A.; McNae, I. W.; Alexeev, D.; Sawyer, L.; Campopiano, D. J., 2004, Acta Cryst., 60(11), 2009-2012, DOI: 10.1107/S0907444904020530.
Assembly of an oxo zirconium (IV) Cluster in a protein cleft.
Zhong, W.; Alexeev, D.; Harvey, I.; Guo, M.; Hunter, D. J. B.; Zhu, H.; Campopiano, D. J.; & Sadler, P. J., Angew. Chem. Int. Ed., 2004, 43(44), 5914-5918, DOI: 10.1002/anie.200460806.
2003
A novel protein-mineral interface.
Alexeev, D.; Zhu, H.; Guo, M.; Zhong, W.; Hunter, D. J. B.; Yang, W.; Campopiano, D. J.; Sadler, P. J., Nat. Struc. Biol., 2003, 10, 297-302, DOI: 10.1038/nsb903.
Oxo-iron clusters in a bacterial iron-trafficking protein: new roles for a conserved motif.
Zhu, H.; Alexeev, D.; Hunter, D. J. B.; Campopiano, D. J.; Sadler, P. J., Biochem. J., 2003, 376, 35-41, DOI: 10.1042/bj20031283.
Biotinylation in the hyperthermophile Aquifex aeolicus: Isolation of a cross-linked BPL:BCCP complex.
Clarke, D. J.; Coulson, J.; Baillie, R.; Campopiano, D. J., Eur. J. Biochem., 2003, 270, 1277-1287, DOI: 10.1046/j.1432-1033.2003.03493.x.
The mechanism of 7,8-diaminopelargonate synthase; the role of the S-adenosylmethionine as the amino donor.
Breen, R. S.; Campopiano, D. J.; Webster, S. W.; Brunton, M.; Watt, R.; Baxter, R. L., Org. Biomol. Chem., 2003, 1, 3498-3499, DOI: 10.1039/B310443P.
Synergistic anion and metal binding to the Ferric-iron Binding Protein from Neisseria gonorrheae (nFBP).
Guo, M.; Yang, W.; Harvey, I.; Coghill, L.; Campopiano, D. J.; Parkinson, J. A.; MacGillivray, R. T. A.; Harris, W. R.; Sadler, P. J., J. Biol. Chem., 2003, 278(4), 2490-2502., DOI: 10.1074/jbc.M208776200.
2002
Purification and characterisation of the BIOH protein of the biotin biosynthetic pathway.
Tomczyk, N. H.; Nettleship, J. E.; Baxter, R. L.; Crichton, H. J.; Webster, S. P.; Campopiano, D. J., FEBS Letts., 2002, 513, 299-304, DOI: 10.1016/S0014-5793(02)02342-6.
2000
Probing the NADPH binding site of Escherichia coli flavodoxin oxidoreductase.
Leadbeater. C.; McIver, L.; Campopiano, D. J.; Webster, S. P.; Baxter, R. L.; Kelly, S. M.; Price, N. C.; Lysek, D. A.; Noble, M. A.; Chapman, S. K.; Munro, A. W., Biochem. J., 2000, 352(2), 257-266, DOI: 10.1042/bj3520257.
Identification of the [Fe-S] cluster-binding residues of Escherichia coli biotin synthase.
McIver, L.; Baxter, R. L.; Campopiano, D. J., J. Biol. Chem., 2000, 275(18), 13888-13894, DOI: 10.1074/jbc.275.18.13888.
Mechanism of 8-Amino-7-oxononanoate Synthase: spectroscopic, kinetic, and crystallographic studies.
Webster, S. P.; Alexeev, D.; Campopiano, D. J.; Alexeeva, M.; Watt, R. M.; Sawyer, L.; Baxter, R. L., Biochemistry, 2000, 39(3), 516-528, DOI: 10.1021/bi991620j.
Mechanistic studies of 8-Amino-7-Oxononanoate Synthase, in Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent proteins. Webster, S. P.; Alexeev, D.; Campopiano, D. J.; Mullan, L. J.; Sawyer, L.; Baxter, R. L., 2000, 135-142 [Iriarte, Kagan, Martinez-Carrion (Eds), Birkhauser Verlag (pubs.)]. ISBN: 978-3-0348-8397-9 (Online).
1999
Flavodoxin (ferrodoxin) NADP+ Oxidoreductase and Flavodoxin – two redox proteins of E. coli in Flavins and Flavoproteins. McIver, L.; Leadbeater, C.; Baxter, R. L.; Campopiano, D. J.; Chapman, S. K.; Munro A. W., 1999, 13, 277-281.
Cubic crystals of chloramphenicol phosphotransferase from Streptomyces venezuelae in complex with chloramphenicol.
Ellis, J.; Campopiano, D. J.; Izard, T., Acta Cryst., 1999, D55, 1086-1088, DOI: 10.1107/S0907444999003029.
1998
Rational design of an inhibitor of dethiobiorin synthetase; interaction of 6-hydroxypyrimidin-4(3H)-one with the adenine base binding site.
Alexeev, D.; Baxter, R. L.; Campopiano, D. J.; McAlpine, R. S.; McIver, L.; Sawyer, L., Tetrahedron, 1998, 54, 15891-15898, DOI: 10.1016/S0040-4020(98)00999-5.
Characterisation of flavodoxin NADP+ oxidoreductase and flavodoxin; key components of electron transfer in Escherichia coli.
McIver, L.; Leadbeater, C.; Campopiano, D. J.; Baxter, R. L.; Daff, S. N.; Chapman, S. K.; Munro, A. W., Eur. J. Biochem., 1998, 257, 577-585, DOI: 10.1046/j.1432-1327.1998.2570577.x.
The crystal structure of 8-Amino-7-oxononanoate Synthase: A bacterial PLP-dependent, acyl-CoA-condensing enzyme.
Alexeev, D.; Alexeeva, M.; Baxter, R. L.; Campopiano, D. J.; Webster, S. P.; Sawyer, L., J. Mol. Biol., 1998, 284, 401-419, DOI: 10.1006/jmbi.1998.2086.
An investigation of flavoprotein redox partners.
McIver, L.; Leadbeater, C.; Campopiano, D. J.; Alexeev, D.; Baxter, R. L., Biochem. Soc. Trans., 1998, 26(3), S271, DOI: 10.1042/bst026s271.
Characterisation of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl CoA condensing enzyme.
Webster, S. P.; Campopiano, D. J.; Alexeev, D.; Alexeeva, M.; Watt, R. M.; Sawyer, L.; Baxter, R. L., Biochem. Soc. Trans., 1998, 26(3), S268. DOI: 10.1042/bst026s268.
1995
Inactivation of chloramphenicol by O-phosphorylation. A novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer.
Mosher, R. H., Camp(opiano), D. J.; Yang, K.; Brown, M. P.; Shaw, W. V.; Vining, L. C., J. Biol. Chem., 1995, 270(45), 27000-27006, DOI: 10.1074/jbc.270.45.27000.
Current Opinion in Chemical Biology
Curr. Opin. Chem. Biol.10.1016/j.cbpa.2019.12.004