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Publications
2023

Our profile on Google Scholar can be found here.


Amine Synthesis Using the Amine Donor N-Phenyl Putrescine (NPP) and the Johnson Matthey Transaminase Biocatalyst Library.
Kimberley C. Dodds, Catherine A. McKenna, Beatriz Dominguez, Andrew L. Lawrence, Dominic J. Campopiano.

DOI: https://doi.org/10.1595/205651323X16843361771150


Versatile Chemo-Biocatalytic Cascade Driven by a Thermophilic and Irreversible α-Oxoamine Synthase. B. Ashley, A. Baslé, M. Sajjad, A. el. Ashram, P. Kelis, J. M.Wright, D. J. Campopiano, ACS Sustain. Chem. Eng. 2023, xxx, xxx-xxx

DOI:
https://doi.org/10.1021/acssuschemeng.3c00243

Publications
2022

Coupled Natural Fusion Enzymes in a Novel Biocatalytic Cascade Convert Fatty Acids to Amines, Shona M. Richardson, Piera M. Marchetti, Michael A. Herrera, and Dominic J. Campopiano, ACS Catal., 2022


DOI: https://doi.org/10.1021/acscatal.2c02954

Characterization of inositol lipid metabolism in gut-associated Bacteroidetes

​Stacey L. Heaver   , Henry H. Le   , Peijun Tang , Arnaud Baslé, Claudia Mirretta Barone , Dai Long Vu   , Jillian L. Waters1 , Jon Marles-Wright, Elizabeth L. Johnson  , Dominic J. Campopiano  and Ruth E. Ley , Nat. Micor. Biol., 2022

DOI: 10.1038/s41564-022-01152-6

Herrera, M.A., Campopiano, D.J. Buy one, get one free. Nat. Synth 1, 420–421 (2022).

https://doi.org/10.1038/s44160-022-00092-7

N-Phenylputrescine (NPP): a natural product inspired amine donor for biocatalysis.
Catherine A. McKenna,a Mária Štiblariková,b Irene De Silvestro,c Dominic J. Campopiano *a and Andrew L. Lawrence *a, Green Chem., 2022


DOI: https://doi.org/10.1039/D1GC02387J

 

npp front cover.jpg

Publications
2021

Convergent evolution of bacterial ceramide synthesis
Stankeviciute. G., Tang. P, Ashley. B, Chamberlain J.D, Hansen. M. E. B, Coleman. A,
D’Emilia. R, Fu. L, Mohan. E. C, Nguyen. Hung, Guan. Z, Campopiano. D. J, Klein. Eric A.​


https://doi.org/10.1038/s41589-021-00948-7

Direct Monitoring of Biocatalytic Deacetylation Reactions by 1H NMR Reveals Fine Details of Substrate Specificity.
De Cesare S., McKenna C. A., Murray L., Bella J., Mullholland N. P. and Campopiano D. J.,Org. Biomol. Chem.,2021 

DOI: https://doi.org/10.1039/D1OB00122A

Porphyromonas gingivalis Sphingolipid Synthesis Limits the Host Inflammatory Response
FG Rocha, ZD Moye, G Ottenberg, P Tang, DJ Campopiano, FC Gibson III, ME Davey, Journal of Dental Research, 2021,
 
 https://doi.org/10.1177/0022034520908784

Publications
2020

D-Phenylglycine aminotransferase  (D-PhgAT) – substrate scope and structural insights of a stereo-inverting biocatalyst used in the preparation of aromatic amino acids.

Scerpio A., De Cesare S., Marles-Wright J., Akhtar M. K., Loake G., Campopiano D. J., Cat. Sci. Technol., 2020.

DOI: https://doi.org/10.1039/D0CY01391A

Harnessing and engineering amide bond forming ligases for the synthesis of amides 
Winn M., Richardson. S. M., Campopiano D.J., Micklefield J., Curr. Opin. Chem. Biol., 2019.

DOI: 10.1016/j.cbpa.2019.12.004

Publications
2019

Synthesis of N-acyl amide natural products using a versatile adenylating biocatalyst 
Marchetti P.M., Richardson. S. M., Kariem N.M., Campopiano D.J., Med. Chem. Comm., 2019.

DOI: 10.1039/C9MD00063A

Use of Isotopically Labelled Substrates Reveals Kinetic Differences Between Human and Bacterial Serine Palmitoyltransferase

Harrison P.J., Gable K., Somashekarappa N., Kelly V., Clarke D.J., Dunn, T.M., Campopiano D.J., J. Lipid Res., 2019.


DOI: 10.1194/jlr.M089367

Publications
2018

Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library
Ekström A.E., Wong J.T., Bella J., Campopiano D.J., Org. Biomol. Chem., 2018, 16, 8144-8149.
 
DOI: 10.1039/C8OB01918E

Sphingolipid biosynthesis in man and microbes.
Harisson P.J., Dunn T.M., Campopiano D.J.; Nat. Prod. Rep., 2018, 35, 921-954.
 
DOI: 10.1039/C8NP00019K

The carbon chain-selective adenylation enzyme TamA: the missing link between fatty acid and pyrrole natural product biosynthesis.

Marchetti, P. M.; Kelly, V.; Simpson, J. P.; Ward, M.; Campopiano, D. J.; Org. Biomol. Chem., 2018, 16, 2735-2740. 

 

DOI: 10.1039/C8OB00441B.

Publications

 

2017

 

 

Enzymes for Biocatalysis: Key Concepts, Engineering Principles and Case Studies.

Sanchez-Carron, G.; Campopiano, D. J., Chapter 18, Pp 442-470. in Contemporary Catalysis: Science, Technology, and Applications. Kamer, P. C. J.; Vogt, D.; Thybaut, J., (Eds.). RSC Publishing, 2017, ISBN: 978-1-84973-990-0.

 

 

Structural evidence for the covalent modification of FabH by 4,5-dichloro-1,2-dithiol-3-one (HR45).

Ekström, A. G.; Kelly, V.; Marles-Wright, J.; Cockroft, S. L.; Campopiano, D. J., Org. Biomol. Chem., 2017, 15, 6310-6313, DOI: 10.1039/c7ob01396e.

 

 

Using the Pimeloyl-CoA Synthetase Adenylation Fold to Synthesise Fatty Acid Thioesters.

Wang, M.; Moynié, L.; Harrison, P. J.; Kelly, V.; Piper, A.; Naismith, J. H.; Campopiano, D. J., Nat. Chem. Biol., 2017, 13(6), 660-667, DOI: 10.1038/nchembio.2361.

 

 

Characterisation of homologous sphingosine 1-phosphate lyase (S1PL) isoforms in the bacterial pathogen Burkholderia pseudomallei.

McLean, C.; Marles-Wright, J.; Custodio, R; Lowther, J.; Kennedy, A. J.; Pollock, J.; Clarke, D. J.; Brown, A. R.; Campopiano, D. J., J. Lipid Res., 2017, 58(1), 137-150. DOI: 10.1194/jlr.M071258.

 

I

L-1beta-induced protection of keratinocytes against Staphylococcus aureus-secreted proteases is mediated by human beta defensin 2.

Wang, B.; McHugh, B.J.; Qureshi, A.; Campopiano, D. J.; Clarke, D. J.; Fitzgerald, J. R.; Dorin, J. R.; Weller, R.; Davidson, D. J., J. Invest. Dermatol., 2017, 137(1), 95-105, DOI: 10.1016/j.jid.2016.08.025.

 

 

2016

 

 

Characterization of secreted sphingosine-1-phosphate lyases required for virulence and intracellular survival of Burkholderia pseudomallei.

Custódio, R.; McLean, C. J.; Scott, A. E.; Lowther, J.; Kennedy, A.; Clarke, D. J.; Campopiano, D. J.; Sarkar-Tyson, M.; Brown, A. R., Mol. Microbiol., 2016, 102(6), 1004-1019, DOI: 10.1111/mmi.13531.

 

 

Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans.

Tuck, L. R.; Altenbach, K.; Ang, T. F.; Crawshaw, A. D.; Campopiano, D. J.; Clarke, D. J.; Marles-Wright, J., Sci. Rep., 2016, 6, 22108, DOI: 10.1038/srep22108.

 

 

Determination of Protein Thiol Reduction Potential by Isotope Labeling and Intact Mass Measurement.

Thurlow, S. E.; Kilgour, D. P.; Campopiano, D. J.; Mackay, C. L.; Langridge-Smith, P. R.; Clarke, D.J.; Campbell, C. J., Anal Chem., 2016, 88(5), 2727-2733, DOI: 10.1021/acs.analchem.5b04195.

 

 

2015

 

Molecular Basis of Streptococcus mutans Sortase A Inhibition by the Flavonoid Natural Product trans-Chalcone.

Wallock-Richards, D. J.; Marles-Wright, J.; Clarke, D.; Maitra, A.; Dodds, M.; Hanley, B.; Campopiano, D. J., Chem. Commun., 2015, 51(52), 10483-10485, DOI: 10.1039/C5CC01816A.

 

Continuous Colorimetric Assay That Enables High-Throughput Screening of N-Acetylamino Acid Racemases.

Sanchez-Carron, G.; Fleming, T.; Holt-Tiffin, K. E.; Campopiano, D. J., Anal. Chem., 2015, 87(7), 3923-3928, DOI: 10.1021/ac5047328.

 

Insights into the Conformations of Three Structurally Diverse Proteins: Cytochrome c, p53, and MDM2, Provided by Variable-Temperature Ion Mobility Mass Spectrometry.

Dickinson, E. R.; Jurneczko, E.; Pacholarz, K. J.; Clarke, D. J.; Reeves, M.; Ball, K. L.; Hupp, T.; Campopiano, D.; Nikolova, P. V.; Barran, P. E., Anal. Chem., 2015, 87(6), 3231-3238. DOI: 10.1021/ac503720v.

 

Desalting large protein complexes during native electrospray mass spectrometry by addition of amino acids to the working solution

Clarke, D. J.; Campopiano, D. J., Analyst, 2015, 140, 2679-2686, DOI: 10.1039/C4AN02334J.

 

2014

 

Garlic Revisited: Antimicrobial Activity of Allicin-Containing Garlic Extracts against Burkholderia cepacia Complex. 

Wallock-Richards, D.; Doherty, C. J.; Doherty, L.; Clarke, D. J.; Place, M.; Govan, J. R.; Campopiano, D. J., PLoS ONE, 2014, 9(12): e112726. DOI:10.1371/journal.pone.0112726.

 

2013

 

Triazole biotin: a tight-binding biotinidase-resistant conjugate. 

Germeroth, A. I.; Hanna, J. R.; Karim, R.; Kundel, F.; Lowther, J.; Neate, P. G.; Blackburn, E. A.; Wear, M. A.; Campopiano, D. J.; Hulme, A. N., Org. Biomol. Chem., 2013, 11(44), 7700-4. DOI :10.1039/c3ob41837e.

 

The chemical basis of serine palmitoyltransferase inhibition by myriocin. 

Wadsworth, J. M.; Clarke, D. J.; McMahon, S. A.; Lowther, J. P.; Beattie, A. E.; Langridge-Smith, P. R.; Broughton, H. B.; Dunn, T. M.; Naismith, J. H.; Campopiano, D. J., J. Am. Chem. Soc., 2013, 135(38), 14276-85. DOI: 10.1021/ja4059876.

 

Reconstitution of the pyridoxal 5'-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis.

Beattie, A. E.; Clarke, D. J.; Wadsworth, J. M.; Lowther, J.; Sin, H. L.; Campopiano, D. J., Chem. Commun., 2013, 49(63), 7058-60. DOI: 10.1039/c3cc43001d.

 

Partial complementation of Sinorhizobium meliloti bacA mutant phenotypes by the Mycobacterium tuberculosis BacA protein. 

Arnold, M. F.; Haag, A. F.; Capewell, S.; Boshoff, H. I.; James, E. K.; McDonald, R.; Mair, I.; Mitchell, A. M.; Kerscher, B.; Mitchell, T. J.; Mergaert, P.; Barry, C. E. 3rd; Scocchi, M.; Zanda, M.; Campopiano, D. J.; Ferguson, G. P., J. Bacteriol., 2013, 195(2), 389-98. DOI: 10.1128/JB.01445-12.

 

The Pyridoxal 5'-Phosphate (PLP)-Dependent Enzyme Serine Palmitoyltransferase (SPT): Effects of the Small Subunits and Insights from Bacterial Mimics of Human hLCB2a HSAN1 Mutations.

Beattie, A.E.; Gupta, S.D.; Frankova, L.; Kazlauskaite, A.; Harmon, J. M.; Dunn, T. M.; Campopiano, D. J., BioMed Res. Int., 2013, 194371, DOI: 10.1155/2013/194371.

 

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature.

Arnison, P. G.; Bibb, M. J.; Bierbaum, G.; Bowers, A. A.; Bugni, T. S.; Bulaj, G.; Camarero, J. A.; Campopiano, D. J.; Challis, G. L.; Clardy, J.; Cotter, P. D.; Craik, D. J.; Dawson, M.; Dittmann, E.; Donadio, S.; Dorrestein, P.C.; Entian, K. D.; Fischbach, M. A.; Garavelli, J. S.; Göransson, U.; Gruber, C.W.; Haft, D. H.; Hemscheidt, T.K.; Hertweck, C.; Hill, C.; Horswill, A. R.; Jaspars, M.; Kelly, W. L.; Klinman, J. P.; Kuipers, O. P.; Link, A. J.; Liu, W.; Marahiel, M.A.; Mitchell, D. A.; Moll, G. N.; Moore, B. S.; Müller, R.; Nair, S. K.; Nes, I. F.; Norris, G. E.; Olivera, B. M.; Onaka, H.; Patchett, M. L.; Piel, J.; Reaney, M. J.; Rebuffat, S.; Ross, R. P.; Sahl, H. G.; Schmidt, E. W.; Selsted, M. E.; Severinov, K.; Shen. B.; Sivonen, K.; Smith, L.; Stein, T.; Süssmuth, R. D.; Tagg, J. R.; Tang, G. L.; Truman, A. W.; Vederas, J. C.; Walsh, C. T.; Walton, J. D.; Wenzel, S. C.; Willey, J. M.; van der Donk, W. A., Nat. Prod. Rep., 2013, 30(1), 108-160, DOI: 10.1039/c2np20085f.

 

2012

 

An Improved Racemase/Acylase Biotransformation for the Preparation of Enantiomerically Pure Amino Acids.

Baxter, S.; Royer, S.; Grogan, G.; Brown, F.; Holt-Tiffin, K. E.; Taylor, I. N.; Fotheringham, I. G.; Campopiano, D. J., J. Am. Chem. Soc., 2012, 134(47), 19310-19313, DOI: 10.1021/ja305438y.

 

Bivalent enzyme inhibitors discovered using dynamic covalent chemistry.

Clipson, A. J.; Bhat, V. T.; McNae, I.; Caniard, A. M.; Campopiano, D. J.; Greaney, M. F., Chemistry, 2012, 18(34) 10562-10570. DOI: 10.1002/chem.201201507.

 

L-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5'-phosphate-thiazolidine adduct.

Lowther, J.; Beattie, A. E.; Langridge-Smith, P. R. R.; Clarke, D. J.; Campopiano, D. J., Med. Chem. Commun., 2012, 3, 1003-1008 DOI: 10.1039/C2MD20020A.

 

Structural, mechanistic and regulatory studies of serine palmitoyltransferase.

Lowther, J.; Naismith, J. H.; Dunn, T. M.; Campopiano, D. J., Biochem. Soc. Trans., 2012, 40(3), 547-554, DOI: 10.1042/BST20110769.

 

Ferric ion (hydr)oxo clusters in the "Venus flytrap" cleft of FbpA: Mössbauer, calorimetric and mass spectrometric studies.

Mukherjee, A.; Bilton, P. R.; Mackay, L.; Janoschka, A.; Zhu, H.; Rea, D.; Langridge-Smith, P. R.; Campopiano, D. J.; Teschner, T.; Trautwein, A. X.; Schünemann, V.; Sadler, P. J., J. Biol. Inorg. Chem., 2012, 17(4), 573-588, DOI: 10.1007/s00775-012-0878-z.

 

2011

 

Role of a conserved arginine residue during catalysis in serine palmitoyltransferase.

Lowther, J.; Charmier, G.; Raman, M. C.; Ikushiro, H.; Hayashi, H.; Campopiano, D. J., FEBS Lett., 2011, 585(12), 1729-1734, DOI: 10.1016/j.febslet.2011.04.013.

 

2010

 

Interaction of human ß-defensin 2 (HBD2) with glycosaminoglycans.

Seo, E. S.; Blaum, B. S.; Vargues, T.; De Cecco, M.; Deakin, J. A.; Lyon, M. ; Barran, P. E.; Campopiano, D. J.; Uhrín, D., Biochemistry, 2010, 49(49), 10486-10495, DOI: 10.1021/bi1011749.

 

The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein.

Raman, M. C.; Johnson, K. A.; Clarke, D. J.; Naismith, J. H.; Campopiano, D. J., Biopolymers, 2010, 93(9) 811-822, DOI: 10.1002/bip.21482.

 

Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.

Lowther, J.; Yard, B. A.; Johnson, K. A.; Carter, L. G.; Bhat, V. T.; Raman, M. C.; Clarke, D. J.; Ramakers, B.; McMahon, S. A.; Naismith, J. H.; Campopiano, D. J., Mol. Biosyst., 2010, 6(9), 1682-1693, DOI: 10.1039/c003743e.

 

Antimicrobial activity of CHIR-090, an inhibitor of lipopolysaccharide biosynthesis, against the Burkholderia cepacia complex.

Bodewits, K.; Raetz, C. R.; Govan J. R.; Campopiano, D. J., Antimicrob. Agents. Chemother., 2010, 54(8), 3531-3533, DOI: 10.1128/AAC.01600-09.

 

Nucleophilic catalysis of acylhydrazone equilibration for protein-directed dynamic covalent chemistry.

Bhat, V. T.; Caniard, A. M.; Luksch, T.; Brenk, R.; Campopiano, D. J.; Greaney, M. F., Nat. Chem., 2010, 2(6), 490-497, DOI: 10.1038/nchem.658.

 

Conformational preferences of linear beta-defensins are revealed by ion mobility-mass spectrometry.

De Cecco, M.; Seo, E. S.; Clarke, D. J.; McCullough, B. J.; Taylor, K.; Macmillan, D.; Dorin, J. R.; Campopiano, D. J.; Barran, P. E., J. Phys. Chem. B, 2010, 114(6). 2312-2318, DOI: 10.1021/jp9111662.

 

Subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins based on catalytic activity.

Clarke, D. J.; Ortega, X. P.; Mackay, C. L.; Valvano, M. A.; Govan, J. R.; Campopiano, D. J.; Langridge-Smith, P.; Brown, A. R., Biochemistry, 2010, 49(6), 1319-1330, DOI: 10.1021/bi901703m.

 

Synthesis and application of a new cleavable linker for "click"-based affinity chromatography.

Landi, F.; Johansson, C. M.; Campopiano, D. J.; Hulme, A. N., Org. Biomol. Chem., 2010, 8(1), 56-59, DOI: 10.1039/b916693a.

 

2009

 

Insights into how nucleotide-binding domains power ABC transport.

Newstead, S.; Fowler, P. W.; Bilton, P.; Carpenter, E. P.; Sadler, P. J.; Campopiano, D. J.; Sansom, M. S.; Iwata, S., Structure, 2009, 9(17), 1213-1222, DOI: 10.1016/j.str.2009.07.009.

 

Dying and necrotic neutrophils are anti-inflammatory secondary to the release of alpha-defensins.

Miles, K.; Clarke, D. J.; Lu, W.; Sibinska, Z.; Beaumont, P. E.; Davidson, D. J.; Barr, T. A.; Campopiano, D. J.; Gray, M., J. Immunol., 2009, 183(3), 2122-2132, DOI: 10.4049/jimmunol.0804187.

 

Efficient production of human beta-defensin 2 (HBD2) in Escherichia coli.

Vargues, T.; Morrison, G. J.; Seo, E. S.; Clarke, D. J.; Fielder, H. L.; Bennani, J.; Pathania, U.; Kilanowski, F.; Dorin, J. R.; Govan, J. R. W.; Mackay, L.; Uhrín, D.; Campopiano, D. J., Protein Pept. Lett., 2009, 16(6), 668-676, DOI: 10.2174/092986609788490122.

 

Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.

Tron, C. M.; McNae, I. W.; Nutley, M.; Clarke, D. J.; Cooper, A.; Walkinshaw, M. D.; Baxter, R. L.; Campopiano, D. J., J. Mol. Biol., 2009, 387(1), 129-146, DOI: 10.1016/j.jmb.2008.12.086.

 

The external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and HSAN1 mutant mimics.

Raman, M. C.; Johnson, K. A.; Yard, B. A.; Lowther, J.; Carter, L. G.; Naismith, J. H.; Campopiano, D. J., J. Biol. Chem., 2009, 284(25), 17328-17339, DOI: 10.1074/jbc.M109.008680.

 

Interrogating the Molecular Details of the Peroxiredoxin Activity of the Escherichia coli Bacterioferritin Comigratory Protein Using High-Resolution Mass Spectrometry.

Clarke, D. J.; Mackay, C. L.; Campopiano. D. J.; Langridge-Smith, P.; Brown, A. R., Biochemistry, 2009, 48(18), 3904-3914, DOI: 10.1021/bi900189e.

 

Preparation of isotopically labelled recombinant beta-defensin for NMR studies.

Seo, E. S.; Vargues, T.; Clarke, D. J.; Uhrín, D.; Campopiano, D. J., Protein Expr. Purif., 2009, 65(2), 179-184, DOI: 10.1016/j.pep.2008.11.007.

 

Contributions of two UDP-glucose dehydrogenases to viability and polymyxin B resistance of Burkholderia cenocepacia.

Loutet, S. A.; Bartholdson, S. J.; Govan, J. R. W.; Campopiano, D. J.; Valvano, M. A.; J. Microb., 2009, 155(6), 2029-2039, DOI: 10.1099/mic.0.027607-0.

 

Effective binding and sensing of lipopolysaccharide: combining complementary pattern recognition receptors.

Ganesh, V.; Bodewits, K.; Bartholdson, S. J.; Natale, D.; Campopiano, D. J.; Mareque-Rivas, J. C., Angew. Chem. Int. Ed., 2009, 48(2), 356-360, DOI: 10.1002/anie.200804168.

 

2008

 

Analysis and separation of residues important for the chemoattractant and antimicrobial activities of beta-defensin 3.

Taylor, K.; Clarke, D. J.; McCullough, B.; Chin, W.; Seo, E.; Yang, D.; Oppenheim, J.; Uhrin, D.; Govan, J. R.; Campopiano, D. J.; MacMillan, D.; Barran, P.; Dorin, J. R., J. Biol. Chem., 2008, 283(11), 6631-6639, DOI: 10.1074/jbc.M709238200.

 

Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step.

Gupta, M.; Caniard, A.; Touceda-Varela, A.; Campopiano, D .J.; Mareque-Rivas, J. C., Bioconjug. Chem., 2008, 19(10), 1964-1967, DOI: 10.1021/bc800273j.

 

Plant host and sugar alcohol induced exopolysaccharide biosynthesis in the Burkholderia cepacia complex.

Bartholdson, S. J.; Brown, A. R.; Mewburn, B. R.; Clarke, D. J.; Fry, S. C.; Campopiano, D. J.; Govan, J. R., Microbiol., 2008, 154, 2513-2521, DOI: 10.1099/mic.0.2008/019216-0

 

2007

 

The Structure of Serine Palmitoyltransferase; Gateway to Sphingolipid Biosynthesis.

Yard, B. A.; Carter, L. G.; Johnson, K. A.; Overton, I. M.; Dorward, M.; Liu, H.; McMahon, S. A.; Oke, M.; Puech, D.; Barton, G. J.; Naismith, J. H.; Campopiano, D. J., J. Mol. Biol., 2007, 370, 870-886, DOI: 10.1016/j.jmb.2007.04.086.

 

A putative gene cluster for aminoarabinose biosynthesis in essential for Burkholderia cenocepacia viability.

Ortega, X. P.; Cardona, S. T.; Brown, A. R.; Loutet, S. A.; Flanagan, R. S.; Campopiano, D. J.; Govan, J. R. W.; Valvano, M. A., J. Bacteriol., 2007, 189, 3639-3644, DOI: 10.1128/JB.00153-07.

 

Maturation of McjA precursor peptide into active microcin MccJ25.

Clarke, D. J.; Campopiano, D. J., Org. & Biomol. Chem., 2007, 5, 2564-2566. DOI: 10.1039/B708478A.

 

Covalent dimer species of beta-defensin Defr1 display potent antimicrobial activity against multidrug-resistant bacterial pathogens.

Taylor, K.; McCullough, B.; Clarke, D. J.; Langley, R. J.; Pechenick, T.; Hill, A.; Polfer, N. C.; Campopiano, D. J.; Barran, P. E.; Dorin, J. E.; Govan, J. R. W., Antimicriob. Agents Chemother., 2007, 51, 1719-1724, DOI: 10.1128/AAC.01531-06.

 

Metals in membranes.

Liang, X.; Campopiano, D. J.; Sadler, P. J., Chem. Soc. Rev., 2007, 36, 968-392, DOI: 10.1039/B617040B. Subject of a RSC Chemical Biology focus article.

 

Histidine ligands in bacterial metallothionein enhance cluster stability.

Blindauer, C. A.; Razi, M. T.; Campopiano, D. J.; Sadler, P. J., J. Biol. Inorg. Chem., 2007, 12, 393-405, DOI: 0. 1007/ s00775-006-0196-4.

 

2006

 

Structural and functional studies of defensin-inspired peptides.

Clarke, D. J.; Campopiano, D. J., Biochem. Soc. Trans., 2006, 34, 251-256, DOI: 10.1042/BST0340251.

 

Discovery of Glutathione S-Transferase inhibitors using dynamic combinatorial chemistry.

Shi, B.; Stephenson, R.; Campopiano, D. J.; Greaney, M. F., J. Am. Chem. Soc., 2006, 128, 8459-8467, DOI: 10.1021/ja058049y.

 

Short Oxo-Titanium(IV) bond in bacterial transferrin: a protein target for metalloantibiotics.

Guo, M.; Harvey, I.; Campopiano, D. J; Sadler, P. J., Angew. Chem. Int. Ed., 2006,  45(17), 2644-2654, DOI: 10.1002/anie.200600260.

 

Suicide inhibition of alpha-oxamine synthases: structures of the covalent adducts of 8-amino-7-oxononanoate synthase with trifluoroalanine.

Alexeev, D.; Baxter, R. L.; Campopiano, D. J.; Kerbarh, O.; Sawyer, L.; Tomczyk, N.; Watt, R.; Webster, S. P., Org. Biomol. Chem., 2006, 4(7), 1209-1212 DOI: 10.1039/B517922J.

 

Mechanism of alpha-oxoamine synthases: identification of the intermediate Claisen product in the 8-amino-7-oxononanoate synthase reaction.

Kerbarh, O.; Campopiano, D. J.; Baxter, R.L., Chem. Commun., 2006, 1, 60-62, DOI: 10.1039/B511837A.

 

Characterisation of DEFB107 by mass spectrometry: Lessons from an anti-antimicrobial defensin.

McCullough, B. J.; Eastwood, H.; Clarke, D. J.; Polfer, N. C.; Campopiano, D. J.; Dorin, J. A.; Maxwell, A.; Langley, R. J.; Govan, J. R. W.; Bernstein, S. L.; Bowers, M. T.; Barran, P. E., Int. J. Mass Spec., 2006, 252, 180-188, DOI: 10.1016/j.ijms.2006.01.054.

 

2005

 

Temperate bacteriophages DK4 and BcepMu from Burkholderia cenocepacia J2315 are unusual, identical and closely associated with the ET12 lineage.

Langley, R. J.; Kervan, D.; Bartholdson, J.; Campopiano, D. J.; Govan, J. R.W., FEMS Microbiol. Letts., 2005, 45, 349-350, DOI: http://dx.doi.org/10.1016/j.femsim.2005.06.001.

 

Is it biologically relevant to measure the structures of small peptides in the gas-phase?

Barran, P. E.; Polfer, N. C.; Campopiano, D. J.; Clarke, D. J.; Langridge-Smith, P. R. R.; Langley, R. J.; Govan, J. R. W.; Maxwell, A.; Dorin, J. R.; Millar, R. P.; Bowers, M. T., Int. J. Mass Spec., 2005, 240, 273-284, DOI: 10.1016/j.ijms.2004.09.013.

 

2004

 

Structure-activity relationships in defensin dimers.

Campopiano, D. J.; Clarke, D. J.; Polfer, N. C.; Barran, P. E.; Langley, R. J.; Govan, J. R. W.; Maxwell, A.; Dorin, J. R., J. Biol. Chem., 2004, 279, 48671-48679, DOI: 10.1074/jbc.M404690200.

 

Cloning, expression, purification, crystallisation and preliminary x-ray characterisation of the full-length single stranded DNA-binding protein from the hyperthermophilic organism Aquifex aeolicus.

Clarke, D. J.; Northey, C. G.; Mack, L. A.; McNae, I. W.; Alexeev, D.; Sawyer, L.; Campopiano, D. J., 2004, Acta Cryst., 60(11), 2009-2012, DOI: 10.1107/S0907444904020530.

 

Assembly of an oxo zirconium (IV) Cluster in a protein cleft.

Zhong, W.; Alexeev, D.; Harvey, I.; Guo, M.; Hunter, D. J. B.; Zhu, H.; Campopiano, D. J.; & Sadler, P. J., Angew. Chem. Int. Ed., 2004, 43(44), 5914-5918, DOI: 10.1002/anie.200460806.

 

2003

 

A novel protein-mineral interface.

Alexeev, D.; Zhu, H.; Guo, M.; Zhong, W.; Hunter, D. J. B.; Yang, W.; Campopiano, D. J.; Sadler, P. J., Nat. Struc. Biol., 2003, 10, 297-302, DOI: 10.1038/nsb903.

 

Oxo-iron clusters in a bacterial iron-trafficking protein: new roles for a conserved motif.

Zhu, H.; Alexeev, D.; Hunter, D. J. B.; Campopiano, D. J.; Sadler, P. J., Biochem. J., 2003, 376, 35-41, DOI: 10.1042/bj20031283.

 

Biotinylation in the hyperthermophile Aquifex aeolicus: Isolation of a cross-linked BPL:BCCP complex.

Clarke, D. J.; Coulson, J.; Baillie, R.; Campopiano, D. J., Eur. J. Biochem., 2003, 270, 1277-1287, DOI: 10.1046/j.1432-1033.2003.03493.x.

 

The mechanism of 7,8-diaminopelargonate synthase; the role of the S-adenosylmethionine as the amino donor.

Breen, R. S.; Campopiano, D. J.; Webster, S. W.; Brunton, M.; Watt, R.; Baxter, R. L., Org. Biomol. Chem., 2003, 1, 3498-3499, DOI: 10.1039/B310443P.

 

Synergistic anion and metal binding to the Ferric-iron Binding Protein from Neisseria gonorrheae (nFBP).

Guo, M.; Yang, W.; Harvey, I.; Coghill, L.; Campopiano, D. J.; Parkinson, J. A.; MacGillivray, R. T. A.; Harris, W. R.; Sadler, P. J., J. Biol. Chem., 2003, 278(4), 2490-2502., DOI: 10.1074/jbc.M208776200.

 

2002

 

Purification and characterisation of the BIOH protein of the biotin biosynthetic pathway.

Tomczyk, N. H.; Nettleship, J. E.; Baxter, R. L.; Crichton, H. J.; Webster, S. P.; Campopiano, D. J., FEBS Letts., 2002, 513, 299-304, DOI: 10.1016/S0014-5793(02)02342-6.

 

2000

 

Probing the NADPH binding site of Escherichia coli flavodoxin oxidoreductase.

Leadbeater. C.; McIver, L.; Campopiano, D. J.; Webster, S. P.; Baxter, R. L.; Kelly, S. M.; Price, N. C.; Lysek, D. A.; Noble, M. A.; Chapman, S. K.; Munro, A. W., Biochem. J., 2000, 352(2), 257-266, DOI: 10.1042/bj3520257.

 

Identification of the [Fe-S] cluster-binding residues of Escherichia coli biotin synthase.

McIver, L.; Baxter, R. L.; Campopiano, D. J., J. Biol. Chem., 2000, 275(18), 13888-13894, DOI: 10.1074/jbc.275.18.13888.

 

Mechanism of 8-Amino-7-oxononanoate Synthase: spectroscopic, kinetic, and crystallographic studies.

Webster, S. P.; Alexeev, D.; Campopiano, D. J.; Alexeeva, M.; Watt, R. M.; Sawyer, L.; Baxter, R. L., Biochemistry, 2000, 39(3), 516-528, DOI: 10.1021/bi991620j.

 

Mechanistic studies of 8-Amino-7-Oxononanoate Synthase, in Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent proteins. Webster, S. P.; Alexeev, D.; Campopiano, D. J.; Mullan, L. J.; Sawyer, L.; Baxter, R. L., 2000, 135-142 [Iriarte, Kagan, Martinez-Carrion (Eds), Birkhauser Verlag (pubs.)]. ISBN: 978-3-0348-8397-9 (Online).

 

1999

 

Flavodoxin (ferrodoxin) NADP+ Oxidoreductase and Flavodoxin – two redox proteins of E. coli in Flavins and Flavoproteins. McIver, L.; Leadbeater, C.; Baxter, R. L.; Campopiano, D. J.; Chapman, S. K.; Munro A. W., 1999, 13, 277-281.

 

Cubic crystals of chloramphenicol phosphotransferase from Streptomyces venezuelae in complex with chloramphenicol.

Ellis, J.; Campopiano, D. J.; Izard, T., Acta Cryst., 1999, D55, 1086-1088, DOI: 10.1107/S0907444999003029.

 

1998

 

Rational design of an inhibitor of dethiobiorin synthetase; interaction of 6-hydroxypyrimidin-4(3H)-one with the adenine base binding site.

Alexeev, D.; Baxter, R. L.; Campopiano, D. J.; McAlpine, R. S.; McIver, L.; Sawyer, L., Tetrahedron, 1998, 54, 15891-15898, DOI: 10.1016/S0040-4020(98)00999-5.

 

Characterisation of flavodoxin NADP+ oxidoreductase and flavodoxin; key components of electron transfer in Escherichia coli.

McIver, L.; Leadbeater, C.; Campopiano, D. J.; Baxter, R. L.; Daff, S. N.; Chapman, S. K.; Munro, A. W., Eur. J. Biochem., 1998, 257, 577-585, DOI: 10.1046/j.1432-1327.1998.2570577.x.

 

The crystal structure of 8-Amino-7-oxononanoate Synthase: A bacterial PLP-dependent, acyl-CoA-condensing enzyme.

Alexeev, D.; Alexeeva, M.; Baxter, R. L.; Campopiano, D. J.; Webster, S. P.; Sawyer, L., J. Mol. Biol., 1998, 284, 401-419, DOI: 10.1006/jmbi.1998.2086.

 

An investigation of flavoprotein redox partners.

McIver, L.; Leadbeater, C.; Campopiano, D. J.; Alexeev, D.; Baxter, R. L., Biochem. Soc. Trans., 1998, 26(3), S271, DOI: 10.1042/bst026s271.

 

Characterisation of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl CoA condensing enzyme.

Webster, S. P.; Campopiano, D. J.; Alexeev, D.; Alexeeva, M.; Watt, R. M.; Sawyer, L.; Baxter, R. L., Biochem. Soc. Trans., 1998, 26(3), S268. DOI: 10.1042/bst026s268.

 

1995

 

Inactivation of chloramphenicol by O-phosphorylation. A novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer.

Mosher, R. H., Camp(opiano), D. J.; Yang, K.; Brown, M. P.; Shaw, W. V.; Vining, L. C., J. Biol. Chem., 1995, 270(45), 27000-27006, DOI: 10.1074/jbc.270.45.27000.

Current Opinion in Chemical Biology

Curr. Opin. Chem. Biol.10.1016/j.cbpa.2019.12.004

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